Figure 5.

Scrib co-localizes with adherens junctions and gap junctions in the developing myocardium. (A–D) Scrib localizes to cardiomyocyte cell membranes at E10.5, co-localizing with β-catenin (A and B) and connexin-43 (C and D). (E–H) In contrast, Vangl2 is found in the cytoplasm of the ventricular cardiomyocytes, with reduced staining in the cell membrane where there is no evidence of co-localization with β-catenin (E and F) or connexin-43 (G and H). (I–K) Although both Scrib and Vangl2 are found in cardiomyocytes, they do not appear to co-localize at E10.5. The position of the acquired z-axis images (B, D, F, H, and J) are indicated by the horizontal white line on the composite images. (L) Co-immunoprecipitation with Scrib antibody in H9C2 cell lysate confirmed no physical interaction with Vangl2. n = 3 for all experiments. Scale bar = 50 µm.