Table 1.
Analysis of phenotypes of Scrib-depleted embryos at E14.5–E15.5
| Genotype | Tissue specificity | Percentage of embryos with external phenotype | External abnormalities observed | Percentage of embryos with heart defect | Types of cardiac malformation |
|---|---|---|---|---|---|
| Scribf/f;PGK-Cre | Somatic and germ cells | 100 | Gastroschisis Neural tube defects |
100 | VSD, DORV Malpositioning in chest |
| Scribf/f;Wnt1-Cre | Neural crest cells | 25 | Exencephaly | 25 | VSD |
| Scribf/f;GATA5-Cre | Epicardium and left ventricular myocardium | 20 | Axial rotation of the lower spine Gastroschisis | 20 | VSD, DORV Malpositioning in chest |
| Scribf/f;Isl1-Cre | Second heart field | 0 | None | 0 | None |
| Scribf/f;Mlc2v-Cre | Myocardium | 0 | None | 40 | Thin ventricular wall |
| Scribf/f;Nkx2.5-Cre | Cardiac progenitors | 0 | None | 100 | Thin ventricular wall, VSD |
VSD, ventricular septal defect; DORV, double outlet right ventricle.