Table 1. Parameters of the fits shown in Fig. 6.
pH 8.2 global | pH 7.5 global | pH 5.5 global | pH 5.5 free | pH 5.5 free | ||
Color in Fig. 6 | red | red | red | black | black | |
f | pC | 100 | 100 | 100 | 100 | 100 |
s* | 0.45 | 0.45 | 0.45 | 0.45 | 0.45 | |
uT | mV | 25 | 25 | 25 | 25 | 25 |
HC | M | 6.3E–9 | 3.16E–8 | 3.16E–6 | 3.16E–6 | 3.16E–6 |
kCB,1* | 1/(sM) | 7.05E15 | 7.05E15 | 7.05E15 | 7.05E15 | 4.11E4 |
kCB,1 HC | 1/s | 4.46E7 | 2.23E8 | 2.23E10 | 2.23E10 | 0.13 |
kBC* | 1/s | 16092 | 16092 | 16092 | 16092 | 7.8 |
kBO,0* | 1/s | 0.0028 | 0.0028 | 0.0028 | 3.21 | 1.92 |
kOB,0* | 1/s | 0.72 | 0.72 | 0.72 | 0.028 | 0.27 |
κOC | 1/s | 2.7 | 2.7 | 2.7 | 0.34 | 1.22E5 |
κCO* | 1/s | 14500 | 14500 | 14500 | 1.86E7 | 14500 |
Erev, fit | mV | 156 | 115 | 0 | 0 | 0 |
Erev, Nernst | mV | 159 | 118 | 0 | 0 | 0 |
The asterisks denote free parameters which were equal for the global fits at cytosolic pH 8.2, 7.5 and 5.5 (italic numbers). The bold numbers indicate values, which are different for different pH values. There are two “free” fits at pH 5.5. Here, only the non-bold, non-italic parameters were equal to those of the global fit for pH 7.5 and 8.2. Bold numbers are different from those of the global fit. “Erev, fit” is obtained from the intersection of the fitted curves with the voltage axis, “Erev, Nernst” is calculated from the pH gradient (pHext = 5.5).