Figure 1.

The him-6 mutant is proficient for timely loading of RAD-51 and MSH-5 at nascent recombination sites. Immunofluorescence images showing RAD-51 and MSH-5 foci in a him-6 mutant gonad, revealing dynamics of RAD-51 and MSH-5 foci that are similar to wild type (shown in Figure S1). Images show a region of the gonad extending from meiotic prophase entry (left) until the end of the pachytene stage (right). RAD-51 foci are abundant during early pachytene, decline during mid-pachytene, and are nearly absent by late pachytene; faint MSH-5 foci appear during early pachytene, become brighter and more abundant during mid-pachytene, and then reduce in number at late pachytene, where they persist at crossover-designated sites. At right, insets of the indicated fields show that although both RAD-51 and MSH-5 foci are present in the same nuclei, they rarely overlap. Scale bar, 10 μm.