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. 2014 Jul 21;198(1):193–207. doi: 10.1534/genetics.114.161513

Figure 2.

Figure 2

The him-6 mutant is proficient for crossover designation and crossover regulation. (A) Immunofluorescence images of late pachytene nuclei from a him-6 mutant gonad, showing that each nucleus has six GFP::COSA-1 foci, each associated with a comet-like ZHP-3 signal; the him-6 mutant is cytologically indistinguishable from wild type, where GFP::COSA-1 foci reflect designation of a single cytologically differentiated CO site on each homolog pair (Yokoo et al. 2012). (B) Graph showing quantitation of GFP::COSA-1 foci in late pachytene nuclei from wild-type (n = 81) and him-6 (n = 94) worms; error bars indicate standard deviation. (C) GFP::COSA-1 and ZHP-3 immunofluorescence in late pachytene nuclei from a him-18 mutant, which is defective at a late step in crossover formation. Scale bar, 10 μm.