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Deconvoluted LC-MS spectrum of native lysozyme (top; theoretical average mass 14,305 Da; observed average mass 14,306 Da) compared to aged lysozyme containing 27% succinimide variant (bottom; theoretical average mass 14,287 Da; observed average mass 14,287 Da). The mass difference of 18 Da corresponds to the loss of a water (H2O) molecule when aspartic acid is converted into the succinimide.
