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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: J Pharm Sci. 2014 Jul 14;103(10):3033–3042. doi: 10.1002/jps.24074

Figure 5.

Figure 5

Fluorescence images of SDS-PAGE gels showing the specificity of rhodamine-aldehyde labeling for hydrazide protein when the reaction was carried out at pH 2. The sample labeled “Z” contains hydrazide and after treatment with rhodamine-aldehyde the band displays a fluorescence signal. In comparison, the native lysozyme (Asp) and aged lysozyme containing succinimide (Asu), which were also treated with rhodamine-aldehyde showed no detectable fluorescence signal, indicating no measurable background reaction under these conditions.