FIG. 5.

The effects of HFD and PE on mitochondrial activity. SD rats were administered either saline or PE (150 mg/kg/day) in the HFD groups for 8 weeks; the rats were then sacrificed, and liver tissues were collected. Liver ATP content was measured in fresh liver homogenates (A). The level of UCP2 was measured by Western blot analysis (B: Western blot image; C: statistical analysis). Liver mitochondria were collected from tissues, and mitochondrial protein oxidation was measured with a commercial kit (D). The activities of mitochondrial complexes I, II, and IV were analyzed (E). The values are the means±the SEM from at least eight animals. *p<0.05, **p<0.01 versus the relevant control group. UCP2, uncoupling protein 2.