Liver total RNA was subject to RT-PCR for SREBP1c (A), ACC1 (B), PNPLA2/ATGL (F), and epididymal adipose PNPLA3 gene expression was also measured (G). Regression analysis was performed for PNPLA3 with SREBP1c (C), PNPLA3 with ACC1 (D), and SREBP1c with ACC1 (E). Liver PNPLA2 (ATGL) (F) and epididymal adipose PNPLA3 (G) were also measured. For RT-PCR results, values were normalized to GAPDH mRNA for each sample and then expressed as the mean ± SEM (n=5), where the mean of the NC group was set to 1.00. * P < 0.001 vs. NC; † P< 0.05 vs. HC; ‡ P < 0.01 vs. HC; §P < 0.05 vs. HC+4%LE; ¶ P <0.001 vs. HC+4%LE.