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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Lipids. 2014 Aug 29;49(10):987–996. doi: 10.1007/s11745-014-3944-1

Fig. 2. Hydrolysis of labeled PtdCho in lipoproteins.

Fig. 2

The lipoproteins were labeled with [14C]-dipalmitoyl PtdCho as described in Methods. An aliquot of the labeled lipoprotein was then treated with bacterial (B. cereus) sphingomyelinase (SMase) (0.05 units/mg lipoprotein) for 1h to deplete the sphingomyelin. Intact and sphingomyelinase-treated lipoproteins (100 μg protein each) were then incubated with EL-conditioned medium for the indicated period of time and the decrease in PtdCho radioactivity compared to the control (incubated with no EL) was determined. The values shown are mean ± SD of 3 experiments.