Table 3. The yield of each type of transformants in wild type, the sgs1Δ and exo1Δ single mutants and the exo1Δ sgs1Δ double mutant.
First, the numbers of each type of transformation events obtained in wild type in assay 5 were normalized to the total of 100 transformants (Figure 3D). Second, the total number of transformants obtained from each mutant was normalized according to the increased transformation efficiency in each mutant (Figure 3A, assay 5), and finally, yield of each type of transformants in each mutant was calculated using data presented in Figure 3D.
| Transformation event | Assay 5
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| wild type | exo1Δ | sgs1Δ | exo1Δ sgs1Δ | ||||||
| Successful gene targeting | 94 | 116 | 1108 | 43 | |||||
|
| |||||||||
| Aberrant events | Integration next to the targeted region (Int)
|
6 | 2
|
4 | 2
|
42 | 30
|
3057 | 0
|
| Targeted chromosome duplications (TCD)
|
3
|
1
|
6
|
2893
|
|||||
| Illegitimate integrations (Ille) | 1 | 1 | 6 | 164 | |||||
|
| |||||||||
| Total | 100 | 120 | 1150 | 3100 | |||||