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Circular dichroism spectra of the wsMUR-TM_v2 compared with the originally designed wsMUR-TM at buffer condition of 5 mM sodium phosphate, pH = 7.0 at temperature of 25 °C. The two proteins displayed similar spectra. Estimates of helical content obtained using the two spectra are presented in table 1. MRME, mean residue molar ellipticity; wsMUR-TM_v2 = modified (second) variant of the water-soluble human mu opioid receptor transmembrane portion.
