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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Methods. 2014 Apr 2;69(2):137–141. doi: 10.1016/j.ymeth.2014.03.026

Figure 3.

Figure 3

Synthesis-dependent strand annealing (SDSA) mechanism of homologous recombination. As in Figure 2, 5’ ends are resected after the break. In this case, one 3’ single-stranded tail invades homologous sequence in the double-stranded donor (thick lines), which carries some distinguishing characteristic (*). The invading end is extended by DNA polymerase, using the donor as template. The extended strand withdraws and pairs with the other end from the original break. Polymerase and ligase activities complete the repair.