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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Methods. 2014 Apr 2;69(2):137–141. doi: 10.1016/j.ymeth.2014.03.026

Figure 4.

Figure 4

Effect of lig4 mutation on ratio of NHEJ and HR repair. Nucleases for targets in the rosy (ry) gene were introduced into wild type (wt) or lig4 mutant Drosophila embryos by mRNA injection, along with appropriate donor DNAs. New germline ry mutants were recovered among offspring of the injected flies, and they were characterized by molecular analysis as resulting from HR or NHEJ. The data on the left are for ZFNs and double-stranded plasmid donors with several kb of homology to the target and are the average of 11 separate experiments. The data on the right are for a TALEN pair injected with a 111-nucleotide single-stranded oligonucleotide donor. These data are selected from more extensive experiments reported in references [29] and [32].