Fig. 4 a.

to c Estradiol production by human granulosa cells obtained from the follicular fluids of IVF/ICSI patients and cultivated for 72 hours. Granulosa cells from homozygous carriers of the MTHFR 677TT mutation displayed significantly lower estradiol synthesis and release (p < 0.006). This same effect was found for unstimulated (a), FSH-stimulated (b) and LH-stimulated (c) granulosa cells, indicating that the MTHFR-677T effect is not primarily produced through the expression or function of gonadotropin receptors (from 34).