Table 2.
List of strains.
| All yeast strains are derivatives of W303 | |
| K699 | MATa, ade2–1, trp1–1, can1–100, leu2–3,112,his3–11,15, ura3, GAL, psi+ |
| K7606 | MATa, Scc3::HA3::HIS3 |
| K8266 | MATa, Scc1-HA3::HIS3 |
| K11990 | MATa, Scc1-PK6::KanMX |
| K12568 | MATalpha, SMC1-myc18::TRP1 |
| K15024 | MATa, tetR-GFP::LEU2, tetOs::URA3, TRP1::Met3-Cdc20 |
| K15031 | MATa, scc1–73, tetR-GFP::LEU2, tetOs::URA3, TRP1::Met3-Cdc20 |
| K15071 | MATa, scc3–1, tetR-GFP::LEU2, tetOs::URA3, TRP1::Met3-Cdc20 |
| K16524 | MATalpha, GAL1-SCC1::TRP1 at SCC1, leu::Pssc1-SCC1(deletion328–348)-HA3::LEU2 |
| K16525 | MATalpha, GAL1-SCC1::TRP1 at SCC1, leu::Pssc1-SCC1(deletion349–369)-HA3::LEU2 |
| K16526 | MATalpha, GAL1-SCC1::TRP1 at SCC1, leu::Pssc1-SCC1(deletion370–393)-HA3::LEU2 |
| K16677 | MATa, pGAL1–10::TRP, tetR-GFP::LEU2, tetOs::URA3, TRP1::Met3-Cdc20 |
| K16678 | MATa, scc3–1, pGAL1–10-Scc3-HA3::TRP, tetR-GFP::LEU2, tetOs::URA3, TRP1::Met3-Cdc20 |
| K16679 | MATa, smc1–259, pGal1–10-Smc1-myc18::LEU2, tetR-GFP::LEU2, tetOs::URA3, TRP1::Met3-Cdc20 |
| K16680 | MATa, scc1–73, pGAL1–10-Scc1-HA3::LEU2, tetR-GFP::LEU2, tetOs::URA3, TRP1::Met3-Cdc20 |
| K17128 | MATalpha, GAL1-SCC1::TRP1 at SCC1, leu::Pscc1-SCC1(del319–327)-HA3::LEU2 |
| K17696 | MATa, ura::ADH1 promoter-OsTIR1–9myc::URA3 |
| K18154 | MATa ura::ADH1 promoter-OsTIR1–9myc::URA3, Scc1-Pk3-aid::KanMX4 |
| K20316 | MATa, ura::ADH1 promoter-OsTIR1–9myc::URA3, Scc3-PK3-aid::KanMX4 |
| K20783 | MATa, ura::ADH1 promoter-OsTIR1–9myc::URA3, Scc3-PK3-aid::KanMX4, Scc1-HA3::HIS3 |
| K20785 | MATa, Scc3-PK3-aid::KanMX4, Scc1-HA3::HIS3 |
| K20787 | MATa, ura::ADH1 promoter-OsTIR1–9myc::URA3, Scc1-Pk3-aid::KanMX4, Scc3::HA3::HIS |
| K20789 | MATa, Scc1-Pk3-aid::KanMX4, Scc3::HA3::HIS |
| K20791 | MATa, Scc1-Pk3-aid::KanMX4 |
| K20795 | MATa, Scc3-PK3-aid::KanMX4 |
| K20854 | MATa/alpha, ura::ADH1 promoter-OsTIR1–9myc::URA3, SCC1EGFP::HIS3, Scc3-Pk3-aid::KanMX4, Mtw1-RFP:KanMX, ADE2 |
| K21329 | MATa, scc3::NatMX4, leu::Scc3(R370E)-HA3::LEU2, Scc1-PK6::TRP1, Mtw1-RFP:KanMX, ADE2 |
| K21443 | MATa, scc3::NatMX4, leu::Scc3(W389E)-HA3::LEU2, Scc1-PK6::TRP1, Mtw1-RFP:KanMX, ADE2 |
| K21713 | MATa, scc3::NatMX4, leu::Scc3(R291E)-HA3::LEU2, Scc1-PK6::TRP1, Mtw1-RFP:KanMX, ADE2 |
| K21719 | MATa, scc3::NatMX4, leu::Scc3(D373R)-HA3::LEU2, Scc1-PK6::TRP1, Mtw1-RFP:KanMX, ADE2 |
| K21777 | MATa/alpha scc3::NatMX4/WT, leu/leu::LEU2 (YIplac128 integrated), Scc1-PK6::TRP1, Mtw1-RFP:KanMX ADE2 |
| K21815 | MATa/alpha scc3::NatMX4/WT, Scc1-PK6::TRP1, leu::Scc3-HA3::LEU2, Mtw1-RFP:KanMX, ADE2 |
| K21817 | MATa, scc3::NatMX4, leu::Scc3-HA3::LEU2, Scc1-PK6::TRP1, Mtw1-RFP:KanMX, ADE2 |
| K23026 | MATa, scc3::NatMX4, leu::Scc3(R370E, Y371E)-HA3::LEU2, Scc1-PK6::TRP1, Mtw1-RFP:KanMX, ADE2 |
| K23048 | MATa scc3–1 (A249E) |
| K23050 | MATa, ura::ADH1 promoter-OsTIR1–9myc::URA3, Scc3-PK3-aid::KanMX4, Scc1(TEV3)220-HA6::HIS3, leu2::His3p-Gal1/His3p-Gal2/Gal1p-Gal4::Leu2, YEplac112 GAL-NLS2-TEV-NLS |
| K23181 | MATa/alpha, ura::ADH1 promoter-OsTIR1–9myc::URA3, Scc1-Pk3-AID::KanMX4, SCC3EGFP::HIS, Mtw1-RFP:KanMX, ADE2 |
| K23235 | MATa, scc3::NatMX4, leu::Scc3(R291A)-HA3::LEU2, Scc1-PK6::TRP1, Mtw1-RFP:KanMX, ADE2 |
| K23289 | MATa, scc3::NatMX4, leu::Scc3(Y371E)-HA3::LEU2, Scc1-PK6::TRP1, Mtw1-RFP:KanMX, ADE2 |
| K23304 | MATa, scc3::NatMX4, leu::Scc3 (120–1060)HA3::LEU2, Scc1-PK6::TRP1, Mtw1, RFP:KanMX, ADE2 |
| K23306 | MATa/alpha leu::Scc3(306–359delta)HA3::LEU2, Scc1-PK6::TRP1, Mtw1-RFP:KanMX, ADE2 |
| K21815 | MATa/alpha, scc3::NatMX4/WT, Scc1-PK6::TRP1, leu::Scc3-HA3::LEU2, Mtw1-RFP:KanMX, ADE2 |
| K21817 | MATa, scc3::NatMX4, Scc1-PK6::TRP1, leu::Scc3-HA3::LEU2, Mtw1-RFP:KanMX, ADE2 |
| K21818 | MATalpha, scc3::NatMX4, Scc1-PK6::TRP1, leu::Scc3-HA3::LEU2, Mtw1-RFP:KanMX, ADE2 |
| K23276 | MATa/alpha, scc3::NatMX4/WT, leu::Scc3(K372A, K404A, R449A)-HA3::LEU2, Scc1-PK6::TRP1, Mtw1-RFP:KanMX, ADE2 |
| K23277 | MATa, scc3::NatMX4/WT, leu::Scc3(K372A, K404A, R449A)-HA3::LEU2, Scc1-PK6::TRP1, Mtw1-RFP:KanMX, ADE2 |
| K23278 | MATalpha, scc3::NatMX4, leu::Scc3(K372A, K404A, R449A)-HA3::LEU2, Scc1-PK6::TRP1, Mtw1-RFP:KanMX, ADE2 |
| K23279 | MATa/alpha scc3::NatMX4/WT, leu::Scc3(K372E, K404E, R449E)-HA3::LEU2, Scc1-PK6::TRP1, Mtw1-RFP:KanMX, ADE2 |
| K23280 | MATa, scc3::NatMX4, leu::Scc3(K372E, K404E, R449E)-HA3::LEU2, Scc1-PK6::TRP1, Mtw1-RFP:KanMX ADE2 |
| K23281 | MATalpha, scc3::NatMX4, leu::Scc3(K372E, K404E, R449E)-HA3::LEU2, Scc1-PK6::TRP1, Mtw1-RFP:KanMX, ADE2 |
| K23382 | MATa/alpha, SCC1EGFP::HIS3, Scc3-PK3-aid::KanMX4, ADE2 |
| K23385 | MATa/alpha, SCC1-Pk3-aid::KanMX4, SCC3EGFP::HIS3, ADE2 |