Table 1.
Newly developed molecular markers in the qRgls2 region on chromosome 5
| Location * (Mb) | Marker | Forward primer (5′–3′) | Reverse primer (5′–3′) | Annealing temperature (°C) | Type |
|---|---|---|---|---|---|
| 67.05 | G414 | TGCTTCCAAACTCCTCCCTA | GCCTTGAGGGTCACCTTTC | 60 | SSR |
| 67.51 | G520 | CACACCACACCAATGCAAAT | CACAGCCATGTTCAGGTCAG | 60 | SSR |
| 69.40 | B96 | CCCTGGGCGCAAAGCAAAGG | TCAGACGGTAGTGCAAGGCACC | 58 | SSR |
| 70.80 | G386 | CAGCACCCTGCTGGTTATTT | GCGGGTTGAAACCGTAGTAG | 58 | SSR |
| 70.90 | IDP36 | TCCTCCTGGCAGTCTAGGAA | TCCGTTTTGTTCTGTTGTGC | 60 | IDP |
| 71.40 | G350 | ACCTCTTCGACGCAACACTC | CGTCGATGAACCTCCGTAGA | 60 | SSR |
| 72.70 | G366 | GCCTGGAAGCTCGTAGTTGT | GTCCAGTCCGTCCCATAAAA | 60 | SSR |
| 73.05 | G346 | CACAGAAGCGTTTCCTTCG | GCTCTGGCTCTGGTTCTAGC | 58 | SSR |
| 73.11 | DD3 | GTGTTTCGCCTCTGGATTTC | AAAAACTGCGTTGCCAGTCT | 58 | IDP |
| 74.05 | Q22 | GGTGCTCCATTGATTGACCT | CGCCCTGTTCTTATTTGCTC | 58 | IDP |
| 74.1 | DD11 | GGAAACAATGGCACACTTCA | GCTTGCATTAGGCTGTTCCT | 58 | SNP |
| 74.50 | G286 | TGTCGTCGTTCCATTACGAG | CGGTTTCCGAAAATGAAGAG | 60 | SSR |
| 74.70 | IDP41 | TGAAGGCTCCAGCTAATGGT | CCGAGGCACGATAAACATCT | 60 | IDP |
| 80.19 | G241 | GATATGGAGGCCCTCTCTCC | ATGATCTCGGTGGTTTCAGG | 58 | SSR |
| 80.41 | G51-1 | ATGCACTGATGGGGAGTGAT | TGTTCTCTGAGCACCAGACG | 60 | SSR |
| 80.83 | G206 | ATCGGCAGATAAAAGCAGGA | CGGGATAAGGGAGGATGATT | 58 | SSR |
| 83.08 | G192 | TTGATGGGCTTAACATTGTCC | TTCGGTTAGGGTGGATTGAG | 60 | SSR |
| 85.87 | G64 | GGAAAAGGGAATGGATGGAT | GGAAGGATCAAGGGAAAGGA | 58 | SSR |
| 95.87 | L19 | AACTCTGGACTCGCTAGGCA | CCGGATGAAGCTAACTGCA | 58 | SSR |
| 131.46 | xl57 | CCGCTCCGCGTATAAAGTAG | CTCTGAAGCCAGGACGGTAG | 60 | SSR |
| 138.12 | G5 | CAATGACTTCCGCACCAGTA | CTCGTTGCCGGTCTCTATGT | 58 | SSR |
| 146.08 | 35-1 | CTCCTTGTTTGGGCCTAATG | TAGGATAAGAGCCCGTGAGC | 60 | SSR |
| 162.00 | xl12 | TCCTCCCTCCCTTGATGAAT | CAGGGAGGCTCAGTAATGGA | 60 | SSR |
*Location: The physical location according to B73 RefGen_v2.