Table 1.
PA chemical analyses of ‘Courier’, ‘Solido’, and ‘LAN3017’ pea seeds and seed coats
| PA analysis using phloroglucinolysis and RP-HPLC-DAD in mature pea seeds | ||||
|---|---|---|---|---|
| Peak ID | Compound | ‘Courier’ | ‘Solido’ | ‘LAN3017’ |
| 3 | GC-P | 29.23 ± 0.73a | 28.99 ± 0.88 | 1.36 ± 0.02 |
| 4 | EGC-P | 55.38 ± 1.05 | 51.16 ± 1.27 | 0.79 ± 0.05 |
| 5 | GC | 9.88 ± 0.29 | 10.53 ± 0.14 | ndb |
| 6 | CT-P isomer | nd | nd | 4.98 ± 0.00 |
| 7 | CT-P | nd | 0.22 ± 0.01 | 21.41 ± 0.01 |
| 8 | EC-P | 0.37 ± 0.01 | 0.60 ± 0.02 | 65.37 ± 0.03 |
| 9 | EGC | 5.13 ± 0.04 | 8.24 ± 0.21 | nd |
| 10 | CT | nd | nd | 0.94 ± 0.00 |
| 11 | EC | nd | 0.27 ± 0.02 | 5.15 ± 0.02 |
| mDP | 6.7 ± 0.2 | 5.3 ± 0.1 | 16.4 ± 0.0 | |
| Conversion yieldc | 83.9 ± 1.6 | 78.3 ± 4.9 | 59.1 ± 0.9 | |
| Total seed PAd | 416.0 ± 7.7 | 264.1 ± 14.6 | 96.7 ± 13.2 | |
| Butanol-HCl quantification of PA content from pea seed coats | ||||
| Total seed coat PA (%)e | 4.57 ± 0.03 | 4.51 ± 0.09 | 5.10 ± 0.07 | |
aMolar % ± SE (n = 2); bnd, not detected; cYield of PA extract calculated.
dTotal seed PA content based on characterized PA subunits, expressed as mg/100 g dry weight of whole seeds. GC-P, gallocatechin-(4α → 2)-phloroglucinol; GC, gallocatechin; EGC-P, epigallocatechin-(4β → 2)-phloroglucinol; EGC, epigallocatechin; CT-P, catechin-(4α → 2)-phloroglucinol; CT, catechin; EC-P, epicatechin-(4β → 2)-phloroglucinol; EC, epicatechin.
eTotal seed coat PA content expressed as % = mg/100 mg dry weight of seed coat sample using 80% methanol extraction. Proanthocyanidin extract from ‘CDC Acer’ pea seed coats purified as described by Jin et al. [41] was used as a standard for the butanol-HCl assay. Data are means ± SE (n = 3).