Skip to main content
. Author manuscript; available in PMC: 2015 Aug 19.
Published in final edited form as: Dev Neurosci. 2014 Aug 19;36(5):409–421. doi: 10.1159/000365074

Figure 1. Perinatal exposure to methadone results in elevated MBP brain expression.

Figure 1

Perinatal exposure to methadone was carried out as indicated under “Methods”. Relative expression levels of the 14, 17, 18.5, and 21.5 kDa MBP splicing variants were analyzed in total brain homogenates at postnatal days 11 and 19. The picture shows a representative western blot. Results in the bar graphs are expressed as % of the controls and are the mean ± SEM from at least 15 brains from 3 different litters/group. Each of the individual values corresponds to the combined scanning of the four MBP isoforms. β-actin levels were used as loading controls. Control vs. methadone, 11 days, p < 0.03; 19 days, p < 0.05. Notice that the intensity of the bands between both ages is not comparable as film exposure times were adjusted to maintain linear detection of the immunoreactivity. Note that the 14 kDa isoform is barely detected at 11 days but significantly increases in the more mature brains at 19 days of age.