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Construction of ΔndhO and OX-ndhO strains and their NdhO levels. A, construct used to generate the ΔndhO mutant. B, construction of a shuttle expression vector, pRL-ndhO, to generate the OX-ndhO mutant. C, Coomassie Brilliant Blue (CBB) staining profiles of total proteins from the ΔndhO, WT (including indicated serial dilutions), and OX-ndhO strains and their immunoblotting using the antibody against NdhO. Total protein corresponding to 3 μg of chlorophyll a was loaded onto each lane.
