FIGURE 3.

NDH-CET activities of ΔndhO, WT, and OX-ndhO strains and their growth. A, monitoring of NDH-CET activity by chlorophyll fluorescence. a.u. is arbitrary units. B, redox kinetics of P700. Cells were illuminated by AL supplemented with FR. After termination of AL illumination, P700⁺ was transiently reduced by electrons from the PQ pool and then reoxidized by background FR. C, kinetics of the P700⁺ re-reduction in darkness after turning off FR in the presence of 10 μm 3-(3,4-dichlorophenyl)-1,1-dimethylurea. The chlorophyll a concentration was adjusted to 20 μg ml⁻¹, and curves are normalized to the maximal signal. D and E, 3 μl of cell suspensions with densities corresponding to A₇₃₀ nm values of 0.1 (upper rows), 0.01 (middle rows), and 0.001 (lower rows) were spotted on agar plates. The plates were incubated under 2% CO₂ in air (v/v) for 6 days at 40 μmol photons m⁻² s⁻¹ (D) and 3 days at 200 μmol photons m⁻² s⁻¹ (E), respectively.