FIGURE 4.

Amounts of Ndh subunits in the thylakoid membranes and NDH-1L and NDH-1M of ΔndhO, WT, and OX-ndhO strains. A, immunodetection of Ndh subunits in thylakoid membranes from the ΔndhO, WT (including indicated serial dilutions), and OX-ndhO strains. Protein blot was performed with antibodies against NdhH, NdhI, NdhK, and NdhM. Lanes were loaded with thylakoid membrane proteins corresponding to 1 μg of chlorophyll a. ATPβ was used as a loading control. B, profiles of BN-PAGE of thylakoid membranes from the WT and mutant strains. Each lane was loaded with thylakoid membrane extract corresponding to 9 μg of chlorophyll a. Red and blue arrows indicate the positions of NDH-1L and NDH-1M complexes, respectively. C, Western analyses of NDH-1L and NDH-1M using the antibodies against NdhH, NdhI, NdhK, and NdhM.