TABLE 1.

Primer sets in this study

Primer	Sequence
Hum T39R-Fwd	5′-CCCCCTCTGGCTCCGCGGCAGTCTCCTTC-3′
Hum T39R-Rev	5′-GAAGGAGACTGCCGCGGAGCCAGAGGGGG-3′
Hum N170K-Fwd	5′-GGTTGATCTTTGCAAGTATGTCTCTGTC-3′
Hum N170K-Rev	5′-GACAGAGACATACTTGCAAAGATCAACC-3′
Hum C330T-Fwd	5′-GATTGTGGATCTCTGCACCTGGAAAGGATTTG-3′
Hum C330T-Rev	5′-CAAATCCTTTCCAGGTGCAGAGATCCACAATC-3′
Hum Q497P-Fwd	5′-GCCCAGGAGCAGGACCAAAGCCTGCTTATC-3′
Hum Q497P-Rev	5′-GATAAGCAGGCTTTGGTCCTGCTCCTGGGC-3′
Hum C106Y-Fwd	5′-CAGAATTTGGCACCTATGCTTTCCCAGATCCC-3′
Hum C106Y-Rev	5′-GGGATCTGGGAAAGCATAGGTGCCAAATTCTG-3′
Hum K297G-Fwd	5′-GCTGACAAAAAAAGGGGAAAGTACCTCAATAATAAATACAG-3′
Hum K297G-Rev	5′-CTGTATTTATTATTGAGGTACTTTCCCCTTTTTTTGTCAGC-3′
Hum L510M-Fwd	5′-CTGAATGCCAAGGACATGAGTGAAGTTGCCCGG-3′
Hum L510M-Rev	5′-CCGGGCAACTTCACTCATGTCCTTGGCATTCAG-3′
Hum S533A-Fwd	5′-GGACTGTTCAAAAAAGCTTGAGCATACTCCAGCAAGC-3′
Hum S533A-Rev	5′-GCTTGCTGGAGTATGCTCAAGCTTTTTTGAACAGTCC-3′
Hum KpnI-Fwd	5′-CAGAATTTGGTACCTGTGCTTTCCCAG-3′
Hum KpnI-Rev	5′-CTGGGAAAGCACAGGTACCAAATTCTG-3′
Hum SalI-Fwd	5′-GGGTTTCTGATTGTCGACCTCTGCTGCTGG-3′
Hum SalI-Rev	5′-CCAGCAGCAGAGGTCGACAATCAGAAACCC-3′
ChkPstI-Fwd	5′-GCAGTTCCCCTGCAGTGACAGATTTAAG-3′
ChkPstI-Rev	5′-CTTAAATCTGTCACTGCAGGGGAACTGC-3′
Chk SmaI-Fwd	5′-GTGAAGTGGCCCGGGCAGAAGTGGAGG-3′
Chk SmaI-Rev	5′-CCTCCACTTCTGCCCGGGCCACTTCAC-3′