FIGURE 10.

Model for pol V Mut regulation by RecA and ATP. Transfer of a RecA molecule (red circle) from RecA* and the subsequent binding of an ATP molecule (inverted blue triangle) to UmuD′₂C form activated pol V Mut (UmuD′₂C-RecA-ATP). A bound ATP molecule is required for polymerase binding to primer-template DNA. pol V copies an undamaged region of DNA, inserts a deoxynucleotide opposite a lesion (X), and continues DNA synthesis beyond the lesion (TLS). pol V Mut is deactivated after dissociating from the primer-template DNA. Dissociation is dependent on pol V Mut-dependent ATP hydrolysis.