FIGURE 4.

OPPC domain is present at the protrusion tips of neuronal cells and the presynaptic area of neuronal synapses. A, distribution of mAb#15 antigen was examined in PC12 cells that were treated with NGF for 48 h (left), in Neuro-2a cells (middle) and in COS-7 cells (right). Immunoreactivities are shown in green. Bar, 30 μm. B, distribution of mAb#15 antigen in adult mouse brain was determined by immunoelectron micrograph analysis visualized by 10-nm gold particles. mAb#15 antigen was located on some of the vesicular structures at the presynaptic area and the perisynaptic sites of the plasma membrane (arrows). Bar, 333 nm.