FIGURE 9.

Lack of evidence for senescence in C-RAF BxB lung tumors upon B-RAF ablation. A, representative pictures of paraffin embedded lung sections from 7-month-old DOX-treated (B-RAFΔ/Δ) and controls animals (B-RAF^flox/flox) that were stained with the indicated markers (brown). B, representative pictures of paraffin-embedded lung sections from 7-month-old DOX-treated (B-RAFΔ/Δ) and control animals (B-RAF^flox/flox) that were immunofluorescence stained for p19^ARF (red), sections were counterstained with DAPI (blue), insets represent segment magnifications of nucleus indicated by arrow. Positive control is a lung section from a 3-week-old SpC-C-RAF BXB11/Bmi1^−/− transgenic mouse, which displays increased expression of p19^ARF (24).