TABLE 2.
Thermal stabilities of various substitutions of residue 2 of 1OPG VLκ and MAK33 VLκ variants
Stabilities against thermal denaturation of the different 1OPG VLκ and MAK33 VLκ residue two variants are shown. Midpoints of the thermal transitions are shown as Tmelt.
| 1OPG VLκ variant | Tmelt | MAK 33 VLκ variant | Tmelt |
|---|---|---|---|
| °C | °C | ||
| 1OPG VLκ-wt | 31.6 ± 0.2 | MAK33 VLκ-WT | 52.0 ± 0.5 |
| OP-E2I | 43.1 ± 0.1 | MK-I2E | 44.0 ± 0.4 |
| OP-E2A | 38.4 ± 0.2 | MK-I2A | 49.2 ± 0.4 |
| OP-E2L | 38.5 ± 0.2 | MK-I2L | 50.0 ± 0.5 |
| OP-E2Q | 35.1 ± 0.2 | MK-I2Q | 46.3 ± 0.4 |
| OP-E2D | 30.3 ± 0.6 | MK-I2D | 42.5 ± 0.4 |
| OP-E2K | 31.3 ± 0.4 | MK-I2K | 43.4 ± 0.5 |