Skip to main content
. 2014 Aug 11;289(39):26847–26858. doi: 10.1074/jbc.M114.590554

FIGURE 3.

FIGURE 3.

Hemin treatment inhibits Th17-mediated responses in colons. A, Western blot analysis of RORγt protein expression in colons extracted from the four groups. β-Actin was used as loading control. B, QRT-PCR analysis of RORγt mRNA in colons isolated from four groups. Data are presented as mean ± S.D. C, QRT-PCR analysis of IL-17A mRNA in colons isolated from four groups. Data are presented as mean ± S.D. D, ELISA analysis of IL-17A in colons isolated from four groups. Data are presented as mean ± S.D. E, qRT-PCR analysis of IL-6 mRNA in colons isolated from four groups. Data are presented as mean ± S.D. F, ELISA analysis of IL-6 in colons isolated from four groups. Data are presented as mean ± S.D. (*, compared with control group, *, p < 0.05; #, compared with DSS group, #, p < 0.05). All results shown are representative of three independent experiments.