FIGURE 5.

Chemical shift changes upon the addition of aptamers to hβ₂m and ΔN6. A, the ¹H-¹⁵N HSQC spectrum of ¹⁵N,¹³C-labeled hβ₂m (60 μm) alone (gray) or in the presence of two molar equivalents of 2′F B6min (magenta). B, expansion of the region boxed in A. C, the ¹H-¹⁵N HSQC spectrum of ¹⁵N,¹³C-labeled hβ₂m (60 μm) alone (gray) or in the presence of two molar equivalents of 2′OH B6min (orange). D, expansion of the region boxed in C. E, the ¹H-¹⁵N HSQC spectrum of ¹⁵N,¹³C-labeled ΔN6 (60 μm) alone (red) or in the presence of two molar equivalents of 2′F B6min (green). F, expansion of the region boxed in E. Chemical shift changes in B, D, and F are annotated with arrows. All spectra were obtained at 25 °C, pH 6.2.