FIGURE 6.
hnRNP-A2/B1 affects steady state levels of ASCL1 mRNA. Kelly neuroblastoma cells were transfected with either control siRNA (si-control) or siRNAs silencing the HNRNPA2B1 (si-hnRNP-A2/B1) or HNRNPR (si-hnRNP-R) genes and grown for 48 h. Cells were further cultivated for an additional 24 h under control (21% O2, gray bars) or hypoxic (1% O2, black bars) conditions. ASCL1 (a), HNRNPA2B1 (b), and HNRNPR (c) mRNA levels after control and specific knockdown conditions. Asterisks indicate significant differences between control and hypoxia for specific siRNA treatment. Number signs indicate a significant alteration by RBP knockdown compared with control si-RNA (n = 6; */#, p < 0.05; **/##, p < 0.01; ***/###, p < 0.001).