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Detergent screen. Sf9 cell membranes were incubated with the indicated detergent for 90 min at 4 °C followed by centrifugation at 100,000 × g for 1 h. A, SDS-PAGE and 1D4 Western blot of pellet (P) and supernatant (S) fractions. B, BN-PAGE and 1D4 Western blot of supernatant fractions from membranes solubilized in fos-choline-12 (Fos-12) and fos-choline-14 (Fos-14). DM, decyl β-maltoside; DDM, dodecyl β-maltoside; OG, octyl β-glucoside.
