FIGURE 3.

Expression of TRPM1 in mammalian cells. HEK cells transiently transfected with TRPM1–1D4 (A) or co-transfected with TRPM1–1D4 and GFP (B) were fixed and permeabilized at 36–42 h post-transfection and labeled with 1D4Ab (A and B) and WGA (A) as a membrane marker. Single optical sections (A) or single slices through the reconstructed volume after deconvolution (B) are shown. Scale bar, 10 μm. C, detergent screen with membranes from transfected HEK cells as described in Fig. 2. Fos-12, fos-choline-12; Fos-14, fos-choline-14; DM, decyl β-maltoside; DDM, dodecyl β-maltoside; OG, octyl β-glucoside; P, pellet; S, supernatant.