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. 2014 Aug 12;289(39):27046–27054. doi: 10.1074/jbc.M114.590851

FIGURE 4.

FIGURE 4.

Characterizes the interaction between Arn-His and H-NS. A, the His pulldown assay under different salt concentrations, with NaCl ranging from 100 mm to 300 mm. F, flow-through; E, eluates from the nickel beads. B, the His-pulldown assay under different pH conditions, from pH 5.5 to 8.5. The buffers used in each experiment are as follows: citrate for pH 5.5, MES for pH 6.5, Hepes for pH 7.5H, Tris for pH 7.5T, and pH 8.5.