FIGURE 5.

An EMSA of H-NS with the DNA mimic protein Arn-His as competition. A, the EMSA result shows that H-NS binds to its own promoter, the hns promoter (32-mer), and H-NS·DNA binding can be antagonized with the DNA mimic protein Arn-His. B, the EMSA result shows that the H-NS/DNA complex is broken only by the addition of Arn-His but not by another DNA mimic protein (i.e. Icp11) or another small and acidic protein (i.e. Neisseria NMO-0455). Group 1: without addition of DNA mimic or small and acidic protein in EMSA; group 2: with addition of Arn-His; group 3: with addition of Icp11; group 4: with addition of NMO-0455. C, H-NS also binds to the early gene promoter p8.1 of the T4 phage, and this DNA-binding property can be disrupted by Arn-His.