FIGURE 10.

Pannexin-1 is more highly expressed in human leukemic leukocytes than in normal human T cells. Left panel, whole cell lysates from Jurkat T cell lymphocytic leukemia, THP1 promonocytic leukemia, or CEM T cell lymphoblastic leukemia were processed for Western blot analysis of Panx1 and actin; * indicates nonspecific immunoreactive band. Right panel, normal human T lymphoblasts were incubated for 4 h with or without 250 ng/ml anti-Fas or 3 μm STS the absence or presence of 100 μm Z-VAD. The cells were processed for Western blot analysis of Panx1 and actin; aliquots of cell lysates from an equivalent number (10⁶) of Jurkat cells were run on the same gel as positive controls.