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Characterization of SpCBM70 and mutants binding to hyaluronan by affinity electrophoresis
a The Kd determined by affinity electrophoresis using a 7-mer binding site, as estimated from the ITC data, was 7.2 ± 0.8 μm, which corresponds to a Ka of 1.4 × 105 m−1.
b NB, no binding.
