. 2014 Aug 6;289(39):27264–27277. doi: 10.1074/jbc.M114.578435

TABLE 6.

Characterization of Hyl by small angle x-ray scattering

Concentration	Molecular mass^a	R_g (Guinier)	R_g (GNOM)	D_max	Porod volume	Particle density^b	Ab initio modeling
Concentration	Molecular mass^a	R_g (Guinier)	R_g (GNOM)	D_max	Porod volume	Particle density^b	χ (DAMMIF)^c	NSD^d
mg/ml	kDa	Å	Å	Å	Å³	g/cm³
Hyl	110.6^e
10.80	146.2	48.2 ± 1.0	49.5	170.5	211,709	0.87	1.93 ± 0.23	0.66 ± 0.02
5.40	143.6	48.4 ± 1.4	49.7	170.1	206,944	0.89	1.60 ± 0.12	0.68 ± 0.02
2.70	149.9	46.7 ± 1.2	48.2	165.6	211,245	0.87	1.64 ± 0.01	0.80 ± 0.04
1.50	155.4	47.2 ± 0.6	48.1	166.6	220,516	0.83	1.43 ± 0.01	0.64 ± 0.02
1.35	134.9	44.7 ± 3.5	46.6	158.1	193,604	0.95	1.44 ± 0.01	0.73 ± 0.03
1.00	144.7	45.1 ± 2.0	46.8	159.5	207,955	0.88	1.32 ± 0.01	0.54 ± 0.06

BSA	66.4^e
1.00	66.5	29.1 ± 0.9	28.8	81	102,486	1.08	NA^f	NA

^a Calculated by the method of Fischer et al. (55).

^b Calculated by the method of Rambo et al. (45), based on a monomeric solution.

^c Average of χ values determined for the 10 models calculated by the DAMMIF ab initio modelling procedure (35).

^d Normalized spatial discrepancies averaged for the 10 models calculated by the DAMMIF ab initio modeling procedure (36).

^e Theoretical molecular masses calculated by primary sequence (20).

^f NA, not applicable.