FIGURE 9.
A USP7 defect impairs the repair of UV light-induced photolesions. Shown is an assessment of CPD repair and 6-4PP repair within genomic DNA of HCT116 and HCT116-USP7−/− cells. The cells were starved in serum-free medium for 24 h, UV-irradiated at 20 J/m2, and allowed to repair DNA for the indicated time periods. Identical amounts of genomic DNA from UV-irradiated cells were subjected to immunoslot-blot analysis of CPD or 6-4PP using the corresponding antibodies. The quantitative data indicate mean ± S.E. from four to six independent immunoslot-blot experiments. A, CPD repair. B, 6-4PP repair.