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. 2014 Aug 13;289(39):27327–27341. doi: 10.1074/jbc.M114.575654

FIGURE 5.

FIGURE 5.

Effect of miR-200a-3p expression in LS-8 cells. A, morphology change of LS-8 cells transduced with pLL-control and pLL-miR-200a lentivirus constructs. B, E-cadherin staining (green fluorescence) of LS-8, pLL control, and pLL-miR-200a transduced cells. DAPI staining used to detect nuclei. C and D, quantitation by real time PCR and Western blot of E-cadherin in LS-8 cells transfected with pLL control (Cont.) vector and PLL-miR-200a, respectively. E and F, quantitation by real time PCR of cyclin D2 and Lef-1 in LS-8 cells transfected with pLL control vector and PLL-miR-200a, respectively. G, as a control, miR-200a expression was increased in pLL-miR-200a-3p-transduced LS-8 cells.