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. Author manuscript; available in PMC: 2015 Aug 26.
Published in final edited form as: Oncogene. 2014 Mar 24;34(9):1073–1082. doi: 10.1038/onc.2014.61

Figure 3.

Figure 3

BCL6 modulates gene expression in breast cancer cells. (A) SK-BR-3 cells were transfected with siRNA to BCL6 (or control). Extracts were then analyzed for BCL6 expression by immunoblot (left) or by ChIP for binding of BCL6, RNA polymerase II, and acetylated histone H4 to region B of the BCL6 gene (right). (B) SK-BR-3 cells treated with siRNA to BCL6 (or control) were analyzed by ChIP for BCL6 (top) and RNA polymerase II (bottom) binding at the indicated sites. Binding is expressed relative to a negative control region. (C) Breast cancer cells treated with the indicated expression vectors and siRNA constructs were transfected with a BCL6 responsive luciferase plasmid and analyzed for luciferase activity. (D) SK-BR-3 breast cancer cells treated with siRNA to BCL6 or control were analyzed by qRT-PCR for expression of the indicated genes relative to GAPDH.