Figure 6.

Macrophages deficient in Vitamin C have reduced antioxidant capacity. Peritoneal macrophages elicited on day 3 following TG-induced peritonitis from VitC sufficient ((a)–(d)) and deficient Gulo^−/− ((e)–(h)) mice were exposed to 12.5, 25, and 50 μM H₂O₂ for 18 hours and probed with MitoTracker Red CMXRos. Macrophages from some VitC deficient mice were incubated with AscA (3 mM, 16 hours) prior to exposure to H₂O₂ followed by staining with MitoTracker Red CMXRos (Deficient + AscA, ((i)–(l))).