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. 2014 Jul 21;25(9):837–843. doi: 10.1089/hum.2014.012

FIG. 2.

FIG. 2.

Tissue PCCA expression levels. (A) Indicated tissues were removed from an untreated Pcca+/+ mouse. Protein content was analyzed by BCA assay, and 50 μg of protein from each tissue was run on SDS-PAGE gels, blotted onto PVDF membrane, and then probed with anti-PCCA antibody. (B) Indicated tissues were removed from Pcca−/−(A138T) mice 25 days after IV injection of 5×1011 vg of AAV1-MCK-PCCA or AAV8-TTR-PCCA. Protein content was analyzed by BCA assay, and 50 μg of protein from each tissue was run on SDS-PAGE gels, blotted onto PVDF membrane, and then probed with anti-PCCA antibody. Wild-type and Pcca−/−(A138T) control lanes are 50 μg of protein from untreated liver tissue. BCA, bicinchoninic acid; IV, intravenous; PVDF, polyvinylidene fluoride; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis.