Fig. 2.

Electrophoretic mobility shift assay (EMSA) for NF-κB binding in RPMI8226 (“RPMI,” human multiple myeloma cell line) or primary CD138+ cells sorted from Pt. 10, 12 or 13 (as labeled). The NF-κB-specific band is designated “igκ” and the un-bound probe is labeled “Free probe”. Oct-1-DNA binding is used as a loading control. Lanes labeled “V” were treated with 100 nM bortezomib for 4 h prior to harvest. Pt 10 and 13 showing bortezomib-inducible NF-κB activity did not respond to BDD treatment; pt 12, with no bortezomib-inducible Nf-κB activity, achieved a partial response (PR) to BDD