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. 2014 Sep 24;9(9):e107203. doi: 10.1371/journal.pone.0107203

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© 2014 Kamimura et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Hematoxylin and eosin staining was performed on the liver sample before (a), immediately following (b), and 3 h (c) after the liver-targeted hydrodynamic gene delivery in rats, and prior to (d), immediately following (e), and 24 h (f) after the liver-targeted hydrodynamic gene delivery in dogs. Scale bar represents 100 µm. (g) Quantitative measurement of hepatic sinusoidal areas. Three different liver sections from 3 rats (n = 9) and 2 dogs (n = 6) were stained and images were captured. Quantitative analysis was performed using ImageJ software (version 1.6.0_20, National Institutes of Health, USA). The values represent mean ± SD. ** p<0.01, *** p<0.001 by ANOVA and Bonferroni's multiple comparison test.