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. 2014 Sep 24;9(9):e107722. doi: 10.1371/journal.pone.0107722

Figure 1. Human NFκB methylation status.

Figure 1

Representative gels for A) methylation sensitive PCR of bisulfite modified DNA for the NFκB promoter; B) PCR of unmodified genomic DNA; and C) methylation sensitive PCR of bisulfite modified DNA for TSLC1 and CDH1 promoter methylation. D) Bisulfite sequencing for NFκB promoter. ‘L’ lanes indicate molecular weight ladders, ‘u’ lanes indicate PCR using primers specific to unmethylated promoter sequences; ‘m’ lanes indicate PCR using primers specific to methylated promoter sequences; ‘u+’ and ‘m+’ indicate respective positive control PCRs; the H2O lane indicates a PCR control containing no DNA template; open circles indicate unmethylated CpG; dashes indicate that no data was obtained).