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. Author manuscript; available in PMC: 2014 Sep 26.
Published in final edited form as: Sci Signal. 2014 May 6;7(324):ra42. doi: 10.1126/scisignal.2005049

Fig. 5. Yap controls the expression of Cox2 and Mmp7 in vitro and in vivo.

Fig. 5

(A) Representative images of IHC staining for Cox2 and Mmp7 in pancreatic sections from KC, KPC, KYC, and KPYC mice. Scale bar, 100 µm. (B) qRT-PCR analysis of ChIP with antibodies to immunoglobulin G (IgG), polymerase II (Pol II), and Yap on Cox2 and Mmp7 promoter regions that contain (S1 and S2) or do not contain (neg) putative TEAD-binding sites in Yap-reconstituted KPY cells. Data are means ± SD from three independent experiments. (C) Fold proliferation in KPY cells expressing control or Yap vector 3 days after addition of DMSO (control), marimastat (MMP inhibitor, 5 µM), or Celebrex (Cox2 inhibitor, 10 µM). Data are means ± SD from three independent experiments. *P< 0.01, **P< 0.001, ***P< 0.0001, two-tailed t test.