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. Author manuscript; available in PMC: 2014 Sep 26.
Published in final edited form as: Sci Signal. 2013 Sep 3;6(291):ra77. doi: 10.1126/scisignal.2004060

Fig. 4. Amot promotes Yap dephosphorylation and antagonizes Yap-Lats1 interaction.

Fig. 4

(A) Western blotting analysis for Yap, p-Yap (at Ser127), or Amot in cytoplasmic and nuclear fractions of HEK293 cells transfected with Amot shRNAs (sh-Amot), a shRNA vector control (Ctrl), Amot-p80, or Amot-p130. Tubulin was used as the cytoplasmic marker and lamin A/C as the nuclear marker. (B) Western blotting analysis of Amotflox MEFs treated with Adeno (−) or Adeno-Cre (+) virus using Amot, p-Yap, and Yap antibodies as indicated. “1” and “2” represent MEFs from two independent embryos. (C) Immunoprecipitation (IP) with the V5-tag antibody and Western blotting analysis to assess the Yap-Lats1 interaction in HEK293 cells cotransfected with V5-Yap and Myc-Lats1 in the presence of vector control, Flag-Amot-p130, or Amot shRNAs. Western blotting data in (A) and (C) are representative of three independent experiments.