Figure 5.

Effects of long-term RANKL treatment on micro-mass chondrocyte cultures. Micro-mass pellets from limb buds of WT mice were cultured in chondrocyte-inducing medium and treated with BMP2±RANKL for 8–24 days, fixed, paraffin embedded, and sectioned. A) and B) H&E/alcian blue-stained sections and type-2 and type-10 collagen (Col) in-situ hybridization (magnification x2 and x20, respectively) at different days of micro-mass cultures. Proliferative chondrocytes are indicated by black arrowheads and hypertrophic chondrocytes are indicated by red arrows in B.