Figure 1.

Human iPSCs Were Labeled with GFP, and They Differentiated into Neural Precursors

(A) hiPSCs were labeled with GFP by a piggyBac vector.

(B) GFP-labeled hiPSCs retained GFP signals after neural induction.

(C) hiPSC-derived neural precursors exhibited immunoreactivities for NESTIN (neural precursor marker), GFAP (astroglial or radial-glial marker), GLT1/ALDH1 (functional/mature astrocyte marker), A2B5/CNPasae (oligodendrocyte lineage marker), and TUJ1/MAP2 (neural lineage marker).

(D) Quantification of hiPSC differentiation in (C).

Data represent mean ± SD (n = 3 experiments). Scale bars, 200 μm.