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. 2014 Sep 11;5:4760. doi: 10.1038/ncomms5760

Figure 6. Splicing inclusion of CHEK1 exon 3 decreases following joint depletion of Tra2α and Tra2β.

Figure 6

(a) Screenshot35 from the UCSC genome browser showing the CHEK1 gene. Tra2β iCLIP tags are shown at the top in purple, and map predominantly to a known alternative exon (CHEK1 exon 3) that is skipped in one mRNA isoform (shaded grey). Example RNA-seq tracks from control MDA-MB-231 cells and after joint Tra2α and Tra2β protein depletion are shown in green and orange respectively. (b) Splicing inclusion of CHEK1 exon 3 is inhibited by siRNA-mediated Tra2α and Tra2β protein depletion in multiple cell types. Splicing inclusion was monitored by RT–PCR and agarose gel electrophoresis, and shown as a mean PSI value after analysis of three independent samples. Probability (P) values were calculated using an independent two-sample t-test between PSI levels of negative control siRNA-treated cells and TRA2A/B siRNA-treated cells (statistical significance: *P<0.05, **P<0.01, ***P<0.0001). An uncropped gel is shown in Supplementary Fig. 13.